Mycelium-based materials were tested for acoustic purposes. Different fungi were grown on substrates and formed to fit into the available impedance tube for transmission loss (TL) and absorption rate (AR) measurements. Results supported previous findings about mycelia being acoustically appropriate for sound insulation, especially in the spectral region of speech. Absorption rates between 0.1 and 0.6 were measured. Furthermore, thickness appears to be the most important factor. However, large-scale production, design, and lifespan are critical issues, and further experiments are needed to develop industry-level products.